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                  • 当前位置: 代写一篇论文多少钱 > 医学论文 > 尿液标本规范化采集和处理在临床诊疗中的价值

                    尿液标本规范化采集和处理在临床诊疗中的价值

                    时间:2018-12-23 10:43作者:郭野 陈倩 张峰 吴卫
                    本文导读:这是一篇关于尿液标本规范化采集和处理在临床诊疗中的价值的文章,临床上常用冰点渗透压仪测定尿液标本的渗透压值,依据尿液的冰点下降值与溶质的摩尔浓度成比例关系,通过测定溶液冰点时的下降值来计算溶液的渗透压。

                      摘    要: 目的 探讨标本放置时间和温度对尿渗透压检测结果的影响,规范临床标本的留取及保存。方法利用全自动冰点渗透压测定仪测定60例北京协和医院门诊患者的尿液标本。尿液置于室温、4℃、-20℃条件下分装保存,分别于0、2、4、8、12、24、36、48、60、72h各测量一次尿渗透压值,比较渗透压值得变化情况。结果 室温放置时,尿渗透压0h测定结果与8h内测定结果差异均无统计学意义(P>0.05);4℃放置时,尿渗透压0h测定结果与48h内测定结果差异均无统计学意义(P>0.05);-20℃放置时,尿渗透压0h测定结果与72h内测定结果比较差异均无统计学意义(P>0.05)。结论 尿液标本室温放置超过8h会影响渗透压值,室温放置时要在8h内检测;4℃放置时要在48h内检测;-20℃放置时72h内检验结果仍然无显着变化。

                      关键词: 尿渗透压; 放置时间; 保存温度;

                      Abstract: Objective To investigate the influence of storage time and preserving temperature on urine osmotic pressure and standardize clinical specimen collection and preservation.Methods A total of 60 cases urine specimens were determined by freezing point osmometer of Peking union medical college hospital outpatients.The urine specimens were classified and stored in different temperatures and determined at 0,2,4,8,12,24,36,48,60,72 hrespectively.Results There was no significant difference of urine osmotic pressure at the room temperature in 8 hours and at 4 ℃in 48 hours and-20 ℃in 72 hours(P>0.05).Conclusion Urine specimens placed at room temperature for more than 8 hours will affect the measured value.Urine osmotic pressure should be performed in 8 hours at room temperature or 48 hours at 4℃.The urine osmotic pressure will not be affected preserved in 72 hours at 20℃.

                      Keyword:棋牌大全下载安装 urine osmotic pressure; preserving temperature; storage time;

                      NIAOSHENTOUYAZHIZAISHENBING、NIAOBENGZHENG、TANGNIAOBINGDENGJIBINGDEZHENDUANJIBINGQINGJIANCEZHONGJUYOUZHONGYAOZUOYONG[1,2,3]。LINCHUANGSHANGCHANGYONGBINGDIANSHENTOUYAYICEDINGNIAOYEBIAOBENDESHENTOUYAZHI,YIJUNIAOYEDEBINGDIANXIAJIANGZHIYURONGZHIDEMOERNONGDUCHENGBILIGUANXI,TONGGUOCEDINGRONGYEBINGDIANSHIDEXIAJIANGZHILAIJISUANRONGYEDESHENTOUYA[4]。NIAOYEBIAOBENSHIHUANZHEZILIUBIAOBEN,ZAILIUQUSHANGLINCHUANGYICONGXINGJIAOCHA,DUIBIAOBENDELIUQUSHIJIANHEFANGZHIWENDUCHANGWUFAYOUXIAOKONGZHI。JIWANGDEYANJIUDADUOJIZHONGZAIBAODAOSHENTOUYAHEJIBINGDEGUANXI,DANSHIDUIYUNIAOYEBIAOBENFANGZHISHIJIANJIWENDUDUINIAOSHENTOUYAJIANCEJIEGUODEYINGXIANGZHEIYISHIYANSHIJIANCEZHONGDEGUANJIANWENTIHAIWEIJIANBAODAO,YINCIBENYANJIUDUIZHEIYIWENTIJINXINGTANTAO,JIANSHAOYINWEILIUQUBIAOBENBUGUIFANSHANGYINQIDENIAOSHENTOUYAJIANCEWUCHA。

                      1、 材料与方法

                      1.1、 标本来源

                      选取60例2016年3月至2013年4月就诊于北京协和医院门诊的患者尿液标本,尿液渗透压值覆盖高中低水平。
                     

                    尿液标本规范化采集和处理在临床诊疗中的价值
                     

                      1.2 、试剂与仪器

                      MEIGUOBio-RadGONGSINIAOYEHUAXUEZHIKONGPIN(PIHAO:65801),MEIGUOAdvancedGONGSICHANPINBINGDIANSHENTOUYAYIBIAOZHUNPIN(3LA028)。ModeI2020BINGDIANSHENTOUYAYIWEIMEIGUOAdvancedGONGSICHANPIN。

                      1.3 、方法

                      XUANQU60LILAIZIBENYUANMENZHENDENIAOYEBIAOBEN,DIZHONGGAOZHIGE20LI(DIZHI:100~300mOsm;ZHONGZHI:300~500mOsm;GAOZHI:>500 mOsm)。MEIFENBIAOBENFENZHUANGZAI30GESHIGUANZHONGFENBIEZHIYUSHIWEN、4℃、-20℃TIAOJIANXIAFENZHUANGBAOCUN,BINGZUOHAOBIAOJI。YINGYONGQUANZIDONGBINGDIANSHENTOUYACEDINGYI,FENBIEZAI0、2、4、8、12、24、36、48、60、72hGECELIANGYICINIAOSHENTOUYAZHI,MEICIJIANCEJUNJIANGBIAOBENHUIFUSHIWEN,CHONGFENHUNYUNHOUZAIJIANCE。YISHIWEN0hJIEGUOWEIDUIZHAOZU,BIJIAOSHENTOUYAZHIZAIBUTONGWENDUHESHIJIANBAOCUNTIAOJIANXIADEBIANHUAQINGKUANG。QUANZIDONGBINGDIANSHENTOUYACEDINGYITONGGUOBIAOZHUNPINDINGBIAOHOU,MEIRIJINXINGDIZHIHEGAOZHIZHIKONG,TONGGUOHOUJINXINGLINCHUANGYANGBENDECEDING。

                      1.4、 统计学处理

                      CAIYONGSPSS16.0TONGJIRUANJIANBAODUISHUJUJINXINGTONGJIFENXI,SHUJUYIBIAOSHI,JINXINGDANYANGBENDEtJIANYAN,ZUJIANJINXINGPEIDUIFJIANYAN,P<0.05RENWEICHAYIJUYOUTONGJIXUEYIYI。

                      2、 结果

                      2.1、 尿液标本在室温放置时渗透压的变化情况

                      SHIWENTIAOJIANXIA,GAO、ZHONG、DIZHIGEZUBIAOBEN8hNEIDENIAOSHENTOUYAZHIYU0hXIANGBI,CHAYIJUNWUTONGJIXUEYIYI(P>0.05),DANSHIZAISHIWENFANGZHI12hHOU,DIZHIZUHEZHONGZHIZUSHENTOUYAYU0hXIANGBICHAYIJUNYOUTONGJIXUEYIYI(P<0.05),DANSHIGAOZHIZUBIAOBENSHENTOUYACHAYIWUTONGJIXUEYIYI(P>0.05);DANGFANGZHI24hHOU,SHENTOUYAZAIDIZHIZU、ZHONGZHIZUHEGAOZHIZUYU0hBIJIAOCHAYIJUNYOUTONGJIXUEYIYI(P<0.05),JIANBIAO1。NIAOSHENTOUYAZAISHIWENFANGZHISHIXUYAOZAI8hYINEICEDING。

                      表1 室温条件下尿渗透压变化趋势
                    表1 室温条件下尿渗透压变化趋势

                      ZHU:YUTONGZU0hBIJIAO,*P<0.05

                      2.2、 尿液标本在4℃放置时渗透压的变化情况

                      ZAI4℃TIAOJIANXIAFANGZHISHI,48hNEIDENIAOSHENTOUYAZHIGAO、ZHONG、DIZHIGEZUYU0hSHIXIANGBICHAYIJUNWUTONGJIXUEYIYI(P>0.05);60hHOU,DIZHIZUSHENTOUYAYU0hXIANGBICHAYIYOUTONGJIXUEYIYI(P<0.05),DANSHIZAIZHONGZHIZUHEGAOZHIZUBIAOBENSHENCHAYIWUTONGJIXUEYIYI(P>0.05);FANGZHI60hHOU,DIZHIZU、ZHONGZHIZUHEGAOZHIZUSHENTOUYAZHIYU0hBIJIAOCHAYIJUNYOUTONGJIXUEYIYI(P<0.05),JIANBIAO2。NIAOSHENTOUYAZAI4℃TIAOJIANXIAFANGZHISHIXUYAOZAI48hYINEICEDING。

                      2.3、 尿液标本在-20℃放置时渗透压的变化情况

                    棋牌大全下载安装   ZAI-20℃TIAOJIANXIAFANGZHISHI,72hNEIDENIAOSHENTOUYAZHIZAIDIZHIZU、ZHONGZHIZUHEGAOZHIZUYU0hSHIXIANGBICHAYIJUNWUTONGJIXUEYIYI(P>0.05),JIANBIAO3。NIAOSHENTOUYAZAI-20℃TIAOJIANXIAFANGZHISHIXUYAOZAI72hYINEICEDING,JIEGUOJUNBUHUIFASHENGGAIBIAN。

                      表2 4℃条件下尿渗透压变化趋势
                    表2 4℃条件下尿渗透压变化趋势

                    棋牌大全下载安装   ZHU:YUTONGZU0hBIJIAO,*P<0.05

                      表3-20℃条件下尿渗透压变化趋势
                    表3-20℃条件下尿渗透压变化趋势

                      3、 讨论

                    棋牌大全下载安装   ZHENGCHANGJITITIYECUNZAIYIDINGSHENTOUYA,SHENTOUYASHIGOUCHENGJITINEIHUANJINGXIANGDUIWENDINGDEZHONGYAOTIAOJIANZHIYI。DANGTIYEDESHENTOUYAPINGHENGPOHUAISHI,KEYINQITIYEZHONGSHUI、DIANJIEZHIHEYOUJIWUBIANHUA,DAOZHIJITIDEGONGNENGHUODONGYICHANG。NIAOSHENTOUYASHIJINGSHENPAIXIEDAONIAOYENEIQUANBURONGZHIWEILIDEZONGLIANG[5]。NIAOSHENTOUYADAIBIAONIAOYEZHONGRONGZHIDEZHIDIANSHULIANG,YUZHIDIANDEZHONGLEI、DAXIAOJISUODAIDEDIANHEWUGUAN。TAZHUYAOJUEDINGYUNIAOZHONGDIANJIEZHIJINIAOSUDESHUIPING,YINGXIANGNIAOSHENTOUYADEWUZHIZHUYAOSHIJINGTIXINGRONGZHI,TEBIESHILIZIHUADERONGZHIWEILI,BUNENGLIZIHUADEWUZHIJIDAFENZIWUZHIYINGXIANGXIAO[6]。JINNIANLAINIAOSHENTOUYACEDINGZHUJIANSHOUDAORENMENDEZHONGSHI,ZAILINCHUANGSHANGDEYINGYONGRIQUGUANGFAN,CHENGWEIXINDESHIYANSHIJIANCEZHIBIAO,DUIYUDUOZHONGJIBINGDEZHENDUAN、ZHILIAOJIPANDUANYUHOUJUYOUZHONGYAOYIYI[7,8,9,10]。

                      NIAOYEBIAOBENGUIFANHUACAIJIHECHULIDUILINCHUANGZHENDUANHEZHILIAOJUYOUZHONGYAOJIAZHI,YESHIBAOZHANGNIAOYEFENXIZHILIANGDEBIYAOQIANTI[11,12,13]。NIAOYEBIAOBENSHIHUANZHEZILIUBIAOBEN,LINCHUANGYICONGXINGJIAOCHA,SHIYINGXIANGJIANYANZHILIANGDEZHONGYAOYINSU[14,15]。NIAOSHENTOUYAJIANCEZAIYIXIEJICENGYIYUANKAIZHANDEBUDUO,BENYUANZAISHIJIGONGZUOZHONGJINGCHANGSHOUDAOWAIYUANDEBIAOBEN,ZAIBIAOBENYUNSONGGUOCHENGZHONG,BUTONGDEYUNSHUSHIJIANHEBAOCUNTIAOJIANDUINIAOSHENTOUYAJIEGUODEYINGXIANGBINGMEIYOUZHUNQUEDEPANDUANBIAOZHUN。BENYANJIUFENBIETANTAOLENIAOYEBIAOBENZAISHIWEN、4℃HE-20℃TIAOJIANXIABAOCUNSHIJIANDUINIAOSHENTOUYADEYINGXIANG,ZHIZAIWEIBIAOBENSONGJIANDEYOUXIAOXINGTIGONGYIJU。BENSHIYANSHIDESHIWENDINGYIWEI22~28℃FANWEINEI,ZAISHIWENFANGZHI,8hHOUDENIAOSHENTOUYAZHIZAIGAOZHONGDIZHIGEZUYUJIKEJIANCEWUMINGXIANCHAYI,DANSHIZAIFANGZHI12hHOU,ZAIDIZHIZUHEZHONGZHIZUNIAOSHENTOUZHIJUNCHENGXIAJIANGQUSHI,BINGXIANZHEDIYUJIKEZHI,DANSHIZAIGAOZHIZUSHENTOUYAXIAJIANGCHAYIBUMINGXIAN。ZAI4℃TIAOJIANXIAFANGZHISHI,DANGFANGZHISHIJIANYANZHANGDAO60hHOU,NIAOSHENTOUYADIZHIZUYUJIKEJIANCEZHIXIANGBIMINGXIANXIAJIANG,ZHONGZHIZUHEGAOZHIZUSHENTOUYAZHIBIANHUAJUNLVEWEIJIANGDI,DANBINGWUTONGJIXUECHAYI。ZAI-20℃TIAOJIANXIAFANGZHI72hHOU,DIZHIZU、ZHONGZHIZUHEGAOZHIZUSHENTOUYAZHIYU0hXIANGBIWUMINGXIANCHAYI。

                      4 、结论

                    棋牌大全下载安装   ZONGSHANGSUOSHU,ZAINIAOYESHENTOUYACEDINGGUOCHENGZHONG,BENYANJIUDEJIELUNKEYIYOUXIAODIZHIDAOSHIYANSHIJIANLIJIESHOUHEJIANCESUOXUYAOGUANZHUDESHIJIANJIEDIANHEBAOCUNTIAOJIAN。DUIYUCAIJIJIYUNSONGBUHEGEDENIAOYEYANGBENBUYUJIESHOUHEJIANYAN,SHIBAOZHENGJIANYANJIEGUOZHUNQUEDEQIANTI。

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